Steven schwartz chicago3/9/2024 Utilizing molecular dynamics calculations, we simulated 21 well-defined genetic pathogenic cardiac troponin T and tropomyosin variants to establish a baseline of pathogenic changes induced in computational observables. To directly address this challenge, we utilized our all-atom computational model of the human full cardiac thin filament (CTF) to predict how sequence substitutions in CTF proteins might affect structure and dynamics on an atomistic level. Difficulties remain, however, in establishing the pathogenic potential of individual mutations, often limiting the use of genotype in management of affected families. Point mutations within sarcomeric proteins have been associated with altered function and cardiomyopathy development. Computational and biophysical determination of pathogenicity of variants of unknown significance in cardiac thin filament. We also observed clear differences between the myosin II and human cardiac β myosin for ATP hydrolysis as well as predict the effect of the mutation I467T on the chemical step. Our methodologies were able to predict the changes to the dynamics of the recovery stroke as well as predict the pathway of breakdown of ATP to ADP and HPO with the stabilization of the metaphosphate intermediate. ![]() Here, like previously, we generated an unbiased thermodynamic ensemble of reactive trajectories for the chemical step using transition path sampling. This work extends previous studies on myosin II with engineered mutations. We explored the free-energy surface of the transition and investigated the effect of the genetic cardiomyopathy causing mutations R453C, I457T, and I467T on this step using metadynamics. The recovery stroke is considered one of the key steps of the kinetic cycle as it is the conformational rearrangement required to position the active site residues for hydrolysis of ATP and interaction with actin. Human cardiac β myosin undergoes the cross-bridge cycle as part of the force-generating mechanism of cardiac muscle. Investigation of the Recovery Stroke and ATP Hydrolysis and Changes Caused Due to the Cardiomyopathic Point Mutations in Human Cardiac β Myosin. Improved sampling frequency and reactant coordinate distances are highlighted as key factors in MTAN transition-state stabilization. ![]() Heavy bacterial MTANs depart from other heavy enzymes as slowed vibrational modes from the heavy isotope substitution caused improved barrier-crossing efficiency. Specific catalytic interactions more favorable for heavy MTANs include improved contacts to the catalytic water nucleophile and to the adenine leaving group. Computational transition-path sampling studies of and MTANs indicated closer enzyme-reactant interactions in the heavy MTANs at times near the transition state, resulting in an improved reaction coordinate geometry. However, both enzymes revealed rare inverse isotope effects on their chemical steps, with faster chemical steps in the heavy enzymes. Heavy bacterial methylthioadenosine nucleosidases (MTANs from and ) gave normal isotope effects in steady-state kinetics, with slower rates for the heavy enzymes. Heavy enzymes typically show slower rates for their chemical steps. Mass alterations perturb femtosecond protein motions and have been used to study the linkage between fast motions and transition-state barrier crossing. Heavy enzyme isotope effects occur in proteins substituted with H-, C-, and N-enriched amino acids. Proceedings of the National Academy of Sciences of the United States of America, 118(40). Inverse heavy enzyme isotope effects in methylthioadenosine nucleosidases. ![]()
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